Description
JF585, SE (JF585, NHS) is an orange fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. JF585, SE can be used immediately for structured illumination (SIM) and stimulated emission depletion (STED) imaging and could be converted to photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments.
Product information
CAS Number: 1811539-88-4
Molecular Weight: 649.59
Formula: C34H27F4N3O6
Chemical Name: 1-[10-(2-carboxylato-5-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}phenyl)-7-(3,3-difluoroazetidin-1-yl)-9,9-dimethyl-2,9-dihydroanthracen-2-ylidene]-3,3-difluoro-1λ⁵-azetidin-1-ylium
Smiles: CC1(C)C2=CC(C=CC2=C(C2=CC=C(C=C12)N1CC(F)(F)C1)C1=CC(=CC=C1C([O-])=O)C(=O)ON1C(=O)CCC1=O)=[N+]1CC(F)(F)C1
InChiKey: GFNMIANIZWSVQR-UHFFFAOYSA-N
InChi: InChI=1S/C34H27F4N3O6/c1-32(2)25-12-19(39-14-33(35,36)15-39)4-7-22(25)29(23-8-5-20(13-26(23)32)40-16-34(37,38)17-40)24-11-18(3-6-21(24)30(44)45)31(46)47-41-27(42)9-10-28(41)43/h3-8,11-13H,9-10,14-17H2,1-2H3
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Maximum absorption wavelength (λabs)=585 nm, maximum emission wavelength (λem)=609 nm. The JF585-SNAP-tag ligand (24) also functioned as a cellular label. The new HaloTag ligands derived from JF585 and JF635 show a high degree of chromogenicity and fluorogenicity, a critical parameter in advanced imaging experiments.
Products are for research use only. Not for human use.
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