Punicalagin

Punicalagin is a polyphenol ingredient isolated from Pomegranate (Punica granatum L.) or the leaves of Terminalia catappa L.. Punicalagin is a reversible and non-competitive 3CLpro inhibitor and inhibits SARS-CoV-2 replication in vitro. Punicalagin is an anti-hepatitis B virus (HBV) agent and has antioxidant, anti-inflammatory, and anticancer effects. Punicalagin has the potential for the research of COVID-19.

Product information

CAS Number: 65995-63-3

Molecular Weight: 1084.72

Formula: C48H28O30

Chemical Name: (1R,35R,38R,55S)-6,7,8,11,12,23,24,27,28,29,37,43,44,45,48,49,50-heptadecahydroxy-2,14,21,33,36,39,54-heptaoxaundecacyclo[33.20.0.0⁴,⁹.0¹⁰,¹⁹.0¹³,¹⁸.0¹⁶,²⁵.0¹⁷,²².0²⁶,³¹.0³⁸,⁵⁵.0⁴¹,⁴⁶.0⁴⁷,⁵²]pentapentaconta-4(9),5,7,10,12,16(25),17(22),18,23,26,28,30,41(46),42,44,47,49,51-octadecaene-3,15,20,32,40,53-hexone

Smiles: OC1O[C@@H]2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C4C5C6=C(C(C7=C(C=C(O)C(O)=C7O)C(=O)O[C@H]2[C@@H]2OC(=O)C7=CC(O)=C(O)C(O)=C7C7=C(C=C(O)C(O)=C7O)C(=O)O[C@@H]12)=C(O)C(O)=C6OC4=O)C(=O)OC=5C(O)=C3O

InChiKey: ZJVUMAFASBFUBG-UYMKNUMKSA-N

InChi: InChI=1S/C48H28O30/c49-10-1-6-17(31(59)27(10)55)19-23-21-22-24(47(70)76-38(21)35(63)33(19)61)20(34(62)36(64)39(22)75-46(23)69)18-9(4-13(52)28(56)32(18)60)43(66)74-37-14(5-72-42(6)65)73-48(71)41-40(37)77-44(67)7-2-11(50)25(53)29(57)15(7)16-8(45(68)78-41)3-12(51)26(54)30(16)58/h1-4,14,37,40-41,48-64,71H,5H2/t14-,37-,40+,41-,48?/m1/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 83.33 mg/mL (76.82 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Punicalagin (100 mg/ml) induces apoptosis in HT-29, HCT116 colon cells. Punicalagin slightly inhibits the PLpro activity with an IC50 of over 50 μM. Punicalagin inhibits the plaque formation of SARS-CoV-2 in a dose-dependent manner, with EC50 values of 7.20 μM. Punicalagin binds at an allosteric site in the dimer interface. Punicalagin inhibit SARS-CoV-2 replication by a mechanism other than preventing S-mediated viral entry.

In Vivo:

Punicalagin (10 mg/kg) inhibits the edema rate of 58.15% in rats.

Products are for research use only. Not for human use.