Description
Sulforhodamine 101 (SR101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Sulforhodamine 101 is used extensively for investigating neuronal morphology and acts as a cell type-selective fluorescent marker of astrocytes both in vivo and in slice preparations.
Product information
CAS Number: 60311-02-6
Molecular Weight: 606.71
Formula: C31H30N2O7S2
Chemical Name: 16-(4-sulfo-2-sulfonatophenyl)-3-oxa-9λ⁵,23-diazaheptacyclo[17.7.1.1⁵,⁹.0²,¹⁷.0⁴,¹⁵.0²³,²⁷.0¹³,²⁸]octacosa-1,4,9(28),13,15,17,19(27)-heptaen-9-ylium
Smiles: [O-]S(=O)(=O)C1=CC(=CC=C1C1C2=CC3CCCN4CCCC(C4=3)=C2OC2C=1C=C1CCC[N+]3CCCC=2C=31)S(O)(=O)=O
InChiKey: COIVODZMVVUETJ-UHFFFAOYSA-N
InChi: InChI=1S/C31H30N2O7S2/c34-41(35,36)20-9-10-21(26(17-20)42(37,38)39)27-24-15-18-5-1-11-32-13-3-7-22(28(18)32)30(24)40-31-23-8-4-14-33-12-2-6-19(29(23)33)16-25(27)31/h9-10,15-17H,1-8,11-14H2,(H-,34,35,36,37,38,39)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 50 mg/mL (82.41 mM; Need ultrasonic). H2O : 8.33 mg/mL (13.73 mM; Need ultrasonic).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Sulforhodamine 101 (SR101) does not label astrocytes in brainstem slices as strong and specific as in the hippocampus or cortex. To minimize excitatory side effects, the concentration of Sulforhodamine 101 has to be kept as low as possible or the labeling procedure can be performed after the actual experiment.
In Vivo:
In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Sulforhodamine 101 or topical application of 100 μM.
Products are for research use only. Not for human use.
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