Hh-Ag1.5, Hedgehog Agonist


Catalog No. size PriceQuantity
M60004-2D 10 mM in DMSO (0.380 mL) $139
M60004-2S 2 mg solid $139
M60004-10S 10 mg solid $563

Description

Product Information
Molecular Weight: 526.04
Formula: C28H26ClF2N3OS
Purity: ≥98%
CAS#: 612542-14-0
Solubility: DMSO up to 50 mM
Chemical Name: 3-chloro-4,7-difluoro-N-(4-(methylamino)cyclohexyl)-N-(3-(pyridin-4-yl)benzyl)benzo[b]thiophene-2-carboxamide
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

 

Biological Activity: 

Hh-Ag1.5 is one of the most potent and selective small-molecule agonists of the Hedgehog pathway, targeting Smoothened (Smo) with an EC50 ~1 nM. Studies showed that it has more potent activity than the recombinant Hh protein in activating Smo and hedgehog pathway.

 

How to Use:

 

  • In vitro: Hh-Ag1.5 was used at 25 nM to induce Hh pathway activation in the reporter gene assay. It was suggested to use at 0.1 µM to replace recombinant Hh protein.
  • In vivo: n/a

 

 

Reference:

  • 1.  Frank-Kamenetsky M, et al. Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. (2002) Journal of Biology (1): 10.2-10.19.
  • 2.  Borzillo GV, et al. The Hedgehog Signaling Pathway as a Target for Anticancer Drug Discovery. (2005) Current Topics in Medicinal Chemistry (5), 147-157.
  • 3.  Pan S, et al. Discovery of NVP-LDE225, a Potent and Selective Smoothened Antagonist. ACS Med. Chem. Lett., 2010, 1 (3), pp 130–134.

 



Products are for research use only. Not for human use.

Payment & Security

American Express Apple Pay Diners Club Discover Elo Google Pay JCB Mastercard PayPal Shop Pay Venmo Visa

Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.

Estimate shipping

You may also like

Recently viewed