Hh-Ag1.5, Hedgehog Agonist

Catalog No. size PriceQuantity
M60004-2D 10 mM in DMSO (0.380 mL) $139
M60004-2S 2 mg solid $139
M60004-10S 10 mg solid $563


Product Information
Molecular Weight: 526.04
Formula: C28H26ClF2N3OS
Purity: ≥98%
CAS#: 612542-14-0
Solubility: DMSO up to 50 mM
Chemical Name: 3-chloro-4,7-difluoro-N-(4-(methylamino)cyclohexyl)-N-(3-(pyridin-4-yl)benzyl)benzo[b]thiophene-2-carboxamide
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.


Biological Activity: 

Hh-Ag1.5 is one of the most potent and selective small-molecule agonists of the Hedgehog pathway, targeting Smoothened (Smo) with an EC50 ~1 nM. Studies showed that it has more potent activity than the recombinant Hh protein in activating Smo and hedgehog pathway.


How to Use:


  • In vitro: Hh-Ag1.5 was used at 25 nM to induce Hh pathway activation in the reporter gene assay. It was suggested to use at 0.1 µM to replace recombinant Hh protein.
  • In vivo: n/a




  • 1.  Frank-Kamenetsky M, et al. Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. (2002) Journal of Biology (1): 10.2-10.19.
  • 2.  Borzillo GV, et al. The Hedgehog Signaling Pathway as a Target for Anticancer Drug Discovery. (2005) Current Topics in Medicinal Chemistry (5), 147-157.
  • 3.  Pan S, et al. Discovery of NVP-LDE225, a Potent and Selective Smoothened Antagonist. ACS Med. Chem. Lett., 2010, 1 (3), pp 130–134.


Products are for research use only. Not for human use.

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