Y-27632 is a widely used, selective inhibitor of the Rho-associated protein kinase p160ROCK with a Ki value at ~ 0.14 μM. In many studies, it was shown and used to enhance the survival and cloning efficiency of many primary cell types after single cell dissociation without affecting their self-renewal properties, including hESC/iPSCs, neural stem cells, intestinal stem cells, and mammary epithelial cells.
CAS Number: 146986-50-7
Molecular Weight: 247.34
Chemical Name: (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide
Appearance: Solid Power.
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO up to 100mM
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined.
HS Tariff Code: 382200
How to use
Y-27632 is typically used at 10 μM concentration in cell culture.
In several hypertension rat models, Y-27632 was orally dosed at 30 mg/kg to significantly decrease blood pressure.
- Uehata M, et al. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension.(1997) Nature. 389(6654):990-4.
- Ishizaki T. et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. (2000) Mol Pharmacol 57, 976-983.
- Koyanagi et al. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. (2008) J. Neurosci Res 86: 270-280.
- Watanabe et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. (2007) Nat Biotech 25: 681-686.
Products are for research use only. Not for human use.
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