DAPT is a widely used γ-secretase inhibitor and serves as an inhibitor of Notch, a γ-secretase substrate. It can also cause a reduction in Aβ40 and Aβ42 levels in human primary neuronal cells (IC50 ~115 nM for total Aβ and ~200 nM for Aβ42) and in brain extracts, as well as in vivo. Since the Notch pathway is involved in development of many cell types, DAPT is used to modulate Notch activity in ESC/iPSC or adult stem cell differentiation studies.
CAS Number: 208255-80-5
Molecular Weight: 432.46
Chemical Name: N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenylglycine-1,1-dimethylethyl ester
Appearance: Solid Power.
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO up to 100 mM
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined.
HS Tariff Code: 382200
How to use
DAPT is used at 10 µM final concentration in cell culture.
DAPT was orally dosed to mice at 100-200 mg/kg once per day or intraperitoneally dosed to mice at 10-100 mg/kg once per day.
- Dovey HF, et al. Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. (2001) J Neurochem. 76(1):173-81.
- Androutsellis-Theotokis A, et al. Notch signalling regulates stem cell numbers in vitro and in vivo. (2006) Nature 442(7104):823-6.
Products are for research use only. Not for human use.
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