Ferrostatin-1 (Fer-1) is a potent inhibitor of ferroptosis with an EC50 ~60 nM, identified by a HTS using erastin-induced ferroptosis in HT-1080 cells. Ferroptosis is a unique iron-dependent form of nonapoptotic cell death triggered by the oncogenic RAS-selective lethal small molecule erastin. Ferroptosis is dependent upon intracellular iron, but not other metals, and is morphologically, biochemically, and genetically distinct from apoptosis, necrosis, and autophagy. Ferrostatin-1 was shown to inhibit ferroptosis in cancer cells, and also glutamate-induced cell death in organotypic rat brain slices. Studies revealed that erastin blocks cystine uptake via inhibition of the cystine/glutamate antiporter, resulting in a defect in the cell’s antioxidative defenses and ultimately leading to an iron-dependent, oxidative cell death (i.e., ferroptosis). Ferrostatin-1 was characterized to prevent erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 serves as a very useful tool to dissect ferroptosis in cancer and many other cell types, and may provide new opportunities to protect tissues and organs from damages resulted from ferroptosis under diseases (such as neurodegeneration).
CAS Number: 347174-05-4
Molecular Weight: 262.35
Chemical Name: ethyl 3-amino-4-(cyclohexylamino)benzoate
Appearance: Solid Power.
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO up to 100mM
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined.
HS Tariff Code: 382200
How to use
Ferrostatin-1 was used at 0.5-2 μM in vitro and cellular assays.
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- Melania Guerrero Hue, et al. FP282 FERROPTOSIS-MEDIATED CELL DEATH IS DECREASED BY CURCUMIN IN RENAL DAMAGE ASSOCIATED TO RHABDOMYOLYSIS, Nephrology Dialysis Transplantation, Volume 34, Issue Supplement_1, June 2019, gfz106.FP282.
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