PluriSIn-1 is a potent small molecule to selectively eliminate human pluripotent stem cells/hPSCs while sparing a large array of tissue-specific stem/progenitor and differentiated cells. PluriSIn-1 was identified by a high-throughput screening against undifferentiated human ESCs. PluriSIn#1 induces ER stress, protein synthesis attenuation, and apoptosis in hPSCs specifically. Mechanistic characterizations uncovered that PluriSIn#1 is a specific inhibitor of stearoyl-coA desaturase (SCD1), the key enzyme in oleic acid biosynthesis, revealing a unique role for lipid metabolism in hPSCs. PluriSIn#1 is also cytotoxic to mouse blastocysts, indicating that the dependence on oleate is inherent to the pluripotent cells. Application of PluriSIn#1 prevented teratoma formation from tumorigenic undifferentiated cells. It can serve as a very useful reagent to enhance the safety of hPSC-based treatments and facilitate safe transplantation of larger cell numbers.
CAS Number: 91396-88-2
Molecular Weight: 213.24
Chemical Name: N'-phenylisonicotinohydrazide
Appearance: Solid Power.
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO up to 100 mM
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥360 days if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined.
HS Tariff Code: 382200
How to use
PluriSIn-1 was used at 10 µM final concentration in culture media for 4 days to eliminate ESCs or iPSCs.
- Ben-David U, et al. Selective Elimination of Human Pluripotent Stem Cells by an Oleate Synthesis Inhibitor Discovered in a High-Throughput Screen. (2013) Cell Stem Cell. In press.
Products are for research use only. Not for human use.
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