OAC1, Oct4 Activator


Catalog No. size PriceQuantity
M60086-2D 10 mM DMSO (0.843 mL) $109
M60086-2S 2 mg solid $109
M60086-10S 10mg solid $447

Description

Product Information
Molecular Weight: 237.26
Formula: C14H11N3O
Purity: ≥ 98%
CAS#: 300586-90-7
Solubility: DMSO up to 100 mM
Chemical Name: N-(1H-pyrrolo[2,3-c]pyridin-5-yl)benzamide
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

 

Biological Activity:

 

OAC1 is a potent and selective small molecule to activate both Oct4 and Nanog promoter-driven luciferase reporter genes, identified from a cell-based high-throughput screening. When added to the reprogramming media along with the four reprogramming factors (Oct4, Sox2, Klf4, and c-Myc), OAC1 can significantly enhance iPSC reprogramming efficiency (20-fold higher than that induced by the 4F alone) and accelerate the reprogramming process. The iPSC colonies derived using OAC1 along with the four factors exhibited typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency in a unique manner, independent of either inhibition of the p53-p21 pathway or activation of the Wnt-β-catenin signaling. OAC1 increases transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. OAC1 may be used for large-scale iPSC generation for various applications.

 


How to Use:

 

 

  • In vitro: OAC1 was used at 1 µM final concentration in iPSC culture media for 7 days (2 days after viral transduction with the four reprogramming factors).
  • In vivo: n/a

 

 

Reference:

  1. Li W, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. (2012) Proc Natl Acad Sci USA.109(51):20853-8.

 

 

Products are for research use only. Not for human use.

 

 

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