ID-8 is a highly potent and selective small molecule inhibitor of dual-specificity tyrosine phosphorylation-regulated kinase (DYRK). It could support Wnt-induced human embryonic stem cell proliferation and survival without differentiation. Using ID-8, a novel and simple chemically defined xeno-free culture system was developed that allows for long-term expansion of human pluripotent stem cells without FGF or TGF-beta pathway activation. These culture conditions do not include xenobiotic supplements, serum, serum replacement, or albumin. Using this culture system, several human pluripotent cell lines maintained pluripotency (>20 passages) and a normal karyotype and still retained the ability to differentiate into derivatives of all three germ layers. This Wnt-dependent culture system should provide a platform for complete replacement of growth factors with chemical compounds.
CAS Number: 147591-46-6
Molecular Weight: 298.29
Chemical Name: 1-(4-methoxyphenyl)-2-methyl-3-nitro-1H-indol-6-ol
Appearance: Solid Power.
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO up to 100 mM
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥360 days if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined.
HS Tariff Code: 382200
How to use
ID-8 was used at 0.5 µM final concentration in cell culture. D-8 (0.1-10 μM) increases human embryonic stem cell (hESC) survival. ID-8 (10 μM) stimulates ESCs proliferation in serum-free media for more than 1 month.
- Hasegawa, K., et al., Wnt signaling orchestration with a small molecule DYRK inhibitor provides long-term xeno-free human pluripotent cell expansion. Stem Cells Transl Med, 2012. 1(1): p. 18-28.
- Miyabayashi, T., et al., Indole derivatives sustain embryonic stem cell self-renewal in long-term culture. Biosci Biotechnol Biochem, 2008. 72(5): p. 1242-8.
Products are for research use only. Not for human use.
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