|Solubility:||DMSO up to 100 mM|
|Storage:||Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.|
FPH1 is a potent and selective small molecule that induced Functional Proliferation of primary human Hepatocytes in vitro, identified by a high-throughput, cell-based screening using primary human hepatocytes. It induced a significant increase in hepatocyte number and elevated the number of hepatocytes undergoing mitosis in a dose-dependent manner. Cells treated with FPH1 also maintained their liver-specific functions. Immunofluorescence staining for Ki67 and albumin, along with FACS-based cell counting, revealed that hepatocytes from a wide range of genetically diverse individuals could be expanded and normal hepatocytes functions could be maintained after expansion. FPH1 can also promote the differentiation of iPS cells toward a hepatic lineage and promote the maturation of induced hepatic cells toward a more adult-like liver phenotype.
How to Use:
- In vitro: FPH1 was suggested to be supplemented into the culture medium on day 1 and 5 at a concentration of 15-20 μM to induce functional proliferation of primary human hepatocytes in vitro.
- In vivo: n/a
- 1. Shan J, et al. Identification of small molecules for human hepatocyte expansion and iPS differentiation. (2013) Nat Chem Biol. 9(8):514-20.
Products are for research use only. Not for human use.