FH1 --- Hepatocytes Functional Enhancer

FH1 is a potent and selective small molecule that enhances hepatocyte functions and promotes the differentiation and maturation of iPSC-derived hepatocytes toward a more mature phenotype in vitro. FH1 was identified by a high-throughput, cell-based screening using primary human hepatocytes. It was added to the basal medium with or without OSM on day 21 after the initiation of differentiation of iPSCs and acted over a period of 9 day to promote more pronounced hepatocyte morphologies, including polygonal cell shapes, visible nuclei and more noticeable bile canaliculi between hepatocytes.

Product information

CAS Number: 2719-05-3

Molecular Weight: 282.34

Formula: C17H18N2O2

Synonym:

BRD-K4477

functional hits 1

Chemical Name: N,N'-(methylenebis(4,1-phenylene))diacetamide

Smiles: CC(=O)NC1C=CC(CC2C=CC(=CC=2)NC(C)=O)=CC=1

InChiKey: OEXMNSOPAKOPEF-UHFFFAOYSA-N

InChi: InChI=1S/C17H18N2O2/c1-12(20)18-16-7-3-14(4-8-16)11-15-5-9-17(10-6-15)19-13(2)21/h3-10H,11H2,1-2H3,(H,18,20)(H,19,21)

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 100 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

FH1 was suggested to be supplemented into the culture medium on day 21 at a concentration of 15-20 μM to enhance hepatocyte functions in vitro over period of 9 days.

References:

1. Shan J, et al. Identification of small molecules for human hepatocyte expansion and iPS differentiation. (2013) Nat Chem Biol. 9(8):514-20.

Products are for research use only. Not for human use.

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