Methoxy-X04 is a fluorescent amyloid β (Aβ) probe for the detection and quantification of plaques, tangles and cerebrovascular amyloid with good specificity. It is a derivative of Congo red and Chrysamine-G that contains no acid groups and is therefore smaller and much more lipophilic than Congo red or Chrysamine-G. Methoxy-X04 retains in vitro binding affinity for amyloid beta (Abeta) fibrils (Ki = 26.8 nM) very similar to that of Chrysamine-G. Using multiphoton microscopy to obtain high-resolution (1 microm) fluorescent images from the brains of living PSI/APP mice, individual plaques could be distinguished within 30 to 60 min after a single IV injection or a single IP injection of Methoxy-X04.
CAS Number: 863918-78-9
Molecular Weight: 344.40
Chemical Name: 4,4'-((1E,1'E)-(2-methoxy-1,4-phenylene)bis(ethene-2,1-diyl))diphenol
Appearance: Solid Power.
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO up to 100 mM
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined.
HS Tariff Code: 382200
How to use
Methoxy-X04 was used at 50 µM final concentration in various in vitro assays.
Methoxy-X04 was administered to mice by a single IV injection of 5 to 10 mg/kg or by a single IP injection of 10 mg/kg to produce high contrast images of plaques and cerebrovascular amyloid in PSI/APP mouse brain.
- Klunk WE, et al. Imaging Abeta plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. (2002) J Neuropathol Exp Neurol. 61(9):797-805.
- Sadowski M, Targeting prion amyloid deposits in vivo. (2004) J Neuropathol Exp Neurol. 63(7):775-84.
- Bolmont T, et al. Dynamics of the microglial/amyloid interaction indicate a role in plaque maintenance. (2008) J Neurosci. 28(16):4283-92.
- Dong J, et al. Multiphoton in vivo imaging of amyloid in animal models of Alzheimer's disease. (2010) Neuropharmacology. 59(4-5):268-75.
Products are for research use only. Not for human use.
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