FLI-06, Notch Pathway Modulator


Catalog No. size PriceQuantity
M60149-2D 10 mM in DMSO (0.456 mL) $139
M60149-2S 2mg solid $139
M60149-10S 10mg solid $570

Description

Product Information
Molecular Weight: 438.52
Formula: C25H30N2O5
Purity: ≥98%
CAS#: n/a
Solubility: DMSO up to 50 mM
Chemical Name: cyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

 

Biological Activity:

FLI-06 is a novel potent and selective small molecule intercepting Notch signaling and the early secretory pathway (EC50 ~2.3 µM), identified by using automated microscopy to monitor the trafficking and processing of a ligand-independent Notch-GFP fusion reporter. FLI-06 can induce accumulation of the reporter at the plasma membrane by interfering with transport in the secretory pathway. It can also disrupt the Golgi apparatus in a manner distinct from that of brefeldin A and golgicide A. FLI-06 inhibited general secretion at a step before exit from the endoplasmic reticulum (ER), which was accompanied by a tubule-to-sheet morphological transition of the ER, rendering FLI-06 the first small molecule acting at such an early stage in secretory traffic. FLI-06 is a very useful chemical probe to study the inhibition of membrane traffic at pre- ER-exit site (ERES) stages without fusion of ER-Golgi. 

How to Use:

  • In vitro:  FLI-06 was used at 10 µM final concentration in vitro and in cellular assays.
  • In vivo:  FLI-06 was applied at 50 μM in E3 embryo medium to zebrafish embryos with chorions torn but not completely removed from the sphere stage until the stage of analysis.

Reference:

  • 1. Krämer A, et al. Small molecules intercept Notch signaling and the early secretory pathway. (2013) Nat Chem Biol. 9(11):731-8.

 


Products are for research use only. Not for human use.

Payment & Security

American Express Apple Pay Diners Club Discover Elo Google Pay JCB Mastercard PayPal Shop Pay Venmo Visa

Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.

Estimate shipping

You may also like

Recently viewed