FLI-06, Notch Pathway Modulator

Catalog No. size PriceQuantity
M60149-2D 10 mM in DMSO (0.456 mL) $139
M60149-2S 2mg solid $139
M60149-10S 10mg solid $570


Product Information
Molecular Weight: 438.52
Formula: C25H30N2O5
Purity: ≥98%
CAS#: n/a
Solubility: DMSO up to 50 mM
Chemical Name: cyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.


Biological Activity:

FLI-06 is a novel potent and selective small molecule intercepting Notch signaling and the early secretory pathway (EC50 ~2.3 µM), identified by using automated microscopy to monitor the trafficking and processing of a ligand-independent Notch-GFP fusion reporter. FLI-06 can induce accumulation of the reporter at the plasma membrane by interfering with transport in the secretory pathway. It can also disrupt the Golgi apparatus in a manner distinct from that of brefeldin A and golgicide A. FLI-06 inhibited general secretion at a step before exit from the endoplasmic reticulum (ER), which was accompanied by a tubule-to-sheet morphological transition of the ER, rendering FLI-06 the first small molecule acting at such an early stage in secretory traffic. FLI-06 is a very useful chemical probe to study the inhibition of membrane traffic at pre- ER-exit site (ERES) stages without fusion of ER-Golgi. 

How to Use:

  • In vitro:  FLI-06 was used at 10 µM final concentration in vitro and in cellular assays.
  • In vivo:  FLI-06 was applied at 50 μM in E3 embryo medium to zebrafish embryos with chorions torn but not completely removed from the sphere stage until the stage of analysis.


  • 1. Krämer A, et al. Small molecules intercept Notch signaling and the early secretory pathway. (2013) Nat Chem Biol. 9(11):731-8.


Products are for research use only. Not for human use.

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