|Solubility:||DMSO up to 10 mM|
|Storage:||Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.|
MHY1485 is a novel potent, selective and cell-permeable mTOR activator. It suppressed the basal autophagic flux, and this inhibitory effect was clearly confirmed in cells under starvation, a strong physiological inducer of autophagy. It can increase cellular mTOR Ser2448 and downstream substrate 4E-BP Thr37/46 phosphorylation level in rat liver Ac2F cells (1 to 2 µM for 1 h). It affects culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes. MHY1485 serves as a novel inhibitor of autophagy with an mTOR activating effect.
How to Use:
- In vitro: MHY1485 was used at 1-5 µM in vitro and cellular assays.
- In vivo: n/a
- 1. Choi YJ, et al. Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion. (2012) PLoS One. 7(8):e43418.
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