|Solubility:||DMSO up to 100 mM|
|Storage:||Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.|
PR-619 is a cell-permeable, non-selective, reversible broad-range inhibitor of the deubiquitinylating enzymes (DUBs) with IC50 of 1-20 μM. PR-619 is shown to increase overall protein polyubiquitination in HEK293T cells in a dose- and time-dependent manner (20 to 150 μM, 0.5 to 20 h) without direct impairment of proteasomal proteolysis. PR-619 treatment results in upregulation of both K48 - and K63-linked polyUb chains. It induces HCT116 cell death with EC50 ~6.3 μM.
How to Use:
- In vitro: PR-619 was used at 10-50 µM final concentration in various in vitro assays.
- In vivo: n/a
- Altun M, et al. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. (2011) Chem Biol. 18(11):1401-12.
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