|Solubility:||DMSO up to 100 mM|
|Chemical Name:||(1r,4r)-N1-(2-benzyl-7-(2-methyl-2H-tetrazol-5-yl)-9H-pyrimido[4,5-b]indol-4-yl)cyclohexane-1,4-diamine hydrochloride|
|Storage:||Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.|
UM171 is a potent small molecule that stimulates the ex vivo expansion of human hematopoietic stem cells/HSC capable of reconstituting human hematopoiesis for at least 6 months in immunocompromised mice. It attenuates cell differentiation and promotes ex vivo expansion of long-term human HSC (LT-HSC) at 35 nM in 11 days fed-batch cultures. UM171’s mechanism is independent of suppression of the aryl hydrocarbon receptor (AHR) like SR1. The properties of UM171 make it a potential candidate for hematopoietic stem cell transplantation and gene therapy.
How to Use:
In vitro: For transplantation experiments, the fed-batch culture system was used. 1x105 CD34+ cord blood (CB) cells/ml were injected into 12 ml or 25 ml bags connected to a syringe loaded pumping system and maintained on an orbital shaker at 37°C and 5% CO2 in air. The pump was set to continuously deliver HSC expansion media supplemented with UM171 (35 nM) at a flow rate of 0.7 μl/min.
In vivo: NSG mice were injected with CD34+ CB cells that had been originally cultured in UM171.
- Fares I, et al. Cord blood expansion. Pyrimidoindole derivatives are agonists of human hematopoietic stem cell self-renewal. (2014) Science. 345(6203):1509-12.
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