Azidothymidine (AZT), Reverse Transcriptase Inhibitor


Catalog No. size PriceQuantity
M60238-2S 2 mg solid $79
M60238-10S 10mg solid $312

Description

Product Information
Molecular Weight: 267.24
Formula: C10H13N5O4
Purity: ≥98%
CAS#: 30516-87-1
Solubility: DMSO up to 100 mM, Water up to 50 mM
Chemical Name: 1-((2R,4S,5S)-4-azido-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

Biological Activity:

Azidothymidine (AZT) is a selective, orally bioavailable and brain penetrant reverse transcriptase inhibitor. It has 100-fold selectivity for HIV reverse transcriptase over DNA polymerase α. It can suppress HIV-1 replication and enhance cell viability in a HIV-1 infected T cell line. It can also suppress growth of a multiple myeloma (MM) cell line and reduces the growth of MM tumor xenografts in mice. In recent studies, AZT can enhance CRISPR-mediated sequence-specific gene knockout via NHEJ in human induced pluripotent stem cells (iPSCs) and other cell types.

How to Use:

In vitro: Azidothymidine (AZT) was used at 5-30 µM final concentration in various in vitro assays.
In vivo: Azidothymidine (AZT) was dosed to mice at 10-50 mg/kg orally once per day.

Reference:

  • 1. Mitsuya H, et al. 3'-Azido-3'-deoxythymidine (BW A509U): an antiviral agent that inhibits the infectivity and cytopathic effect of human T-lymphotropic virus type III/lymphadenopathy-associated virus in vitro. (1985) Proc Natl Acad Sci USA. 82(20):7096-100.
  • 2. Furman PA, et al. Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase. (1986) Proc Natl Acad Sci USA. 83(21):8333-7.
  • 3. Broder S, et al. The development of antiretroviral therapy and its impact on the HIV-1/AIDS pandemic. (2010) Antiviral Res. 85(1):1-18. 
  • 4. Pereira J, et al. Azidothymidine is effective against human multiple myeloma: a new use for an old drug? (2013) Anticancer Agents Med Chem. 13(1):186-92.
  • 5. Yu C, et al. Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells. (2015) Cell Stem Cell 16(2):142-7. 
Products are for research use only. Not for human use. 

Payment & Security

American Express Apple Pay Diners Club Discover Elo Google Pay JCB Mastercard PayPal Shop Pay Venmo Visa

Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.

Estimate shipping

You may also like

Recently viewed