Harmine is a potent, selective and orally bioavailable DYRK1A inhibitor with IC50 of 80 nM. It inhibits phosphorylation of tau directly by DYRK1A (IC50 ~700 nM). It has >10-fold selectivity over DYRK3 and DYRK2 (IC50 ~800 nM and 900 nM respectively. Harmine is also a unique regulator of PPARγ expression that acts by inhibiting the Wnt signalling pathway in a cell-specific manner. It attenuates inflammatory gene expression (TNFα, IL-1β, iNOS) and macrophage accumulation in adipose tissue. Administration of harmine (30 mg/kg) to obese db/db mice resulted in reduced blood glucose, free fatty acids, and triglyceride levels, delayed hyperglycemia, and improved insulin sensitivity. Being function as a new class of human beta cell mitogenic compound, by using three different mouse and human islet in vivo-based models, harmine is able to induce beta cell proliferation, increase islet mass and improve glycemic control. The nuclear factors of activated T cells (NFAT) family of transcription factors are defined as likely mediators of human beta cell proliferation and differentiation.
CAS Number: 442-51-3
Molecular Weight: 212.25
Chemical Name: 7-methoxy-1-methyl-9H-pyrido[3,4-b]indole
Appearance: Solid Power.
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO up to 100 mM
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥360 days if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined.
HS Tariff Code: 382200
How to use
Harmine was used at 10 µM final concentration in various in vitro assays.
Harmine was dosed orally to obese db/db mice at 30 mg/kg. Harmine was dosed by intraperitoneal injection at 10 mg/kg in mouse partial pancreatectomy model (PPX), Euglycemic human islet transplantation model and diabetic marginal mass human islet transplantation model.
- Bain J, et al. The selectivity of protein kinase inhibitors: a further update. (2007) Biochem J. 408(3):297-315.
- Waki H, et al. The small molecule harmine is an antidiabetic cell-type-specific regulator of PPARgamma expression. (2007) Cell Metab. 5(5):357-70.
- Egusa H, et al. The small molecule harmine regulates NFATc1 and Id2 expression in osteoclast progenitor cells. (2011) Bone. 49(2):264-74.
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.