|Solubility:||DMSO and water up to 100 mM|
|Chemical Name:||(S)-methyl 5-guanidino-2-(4-methylphenylsulfonamido)pentanoate, hydrochloride|
|Storage:||Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.|
TAME is a competitive inhibitor of the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). It promotes Cdc20 autoubiquitination and inhibits cyclin proteolysis in mitotic Xenopus oocyte extracts (IC50 ~ 12 μM) and blocks cyclin degradation in interphase extract.
How to Use:
In vitro: TAME was used at 50-200 µM final concentration in various in vitro assays.
In vivo: n/a
- 1. Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. (2010) Cancer Cell. 18(4):382-95.
- 2. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. (2012) Nat Chem Biol. 8(4):383-92.
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.