RS-1, CRISPR Editing Enhancer

Catalog No. size PriceQuantity
M60300-2S 2 mg solid $89
M60300-10S 10 mg solid $356


Product Information
Molecular Weight: 524.23
Formula: C20H16Br2N2O3S
Purity: ≥98%
CAS#: 312756-74-4
Solubility: DMSO up to 100 mM
Chemical Name: 3-(N-benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.


Biological Activity:

RS-1was previously characterized as a RAD51-Stimulatory compound and now found to be an HDR enhancer to enhance CRISPR/Cas9- and TALEN-mediated knock-in efficiency. It increases the knock-in efficiency by two- to five-fold at different loci, whereas NHEJ inhibitor SCR7 has minimal effects. RS-1 was applied for animal production and have achieved multifold improvement on the knock-in rates as well. RS-1 can stimulate the human homologous recombination protein RAD51in various conditions. In PC3 prostate cancer cells, RS-1–induced lethality was accompanied by the formation of microscopically visible RAD51 nuclear protein foci occurring in the absence of any DNA-damaging treatment. Treatment with RS-1 promoted significant antitumor responses in a mouse model.

How to Use:

In vitro: RS-1 was used at 15 µM final concentration in TALEN- or Cas9-mediated knock-in experiments.
In vivo: 
RS-1 was used at 7.5 μM for the in vivo knock-in animal production. RS-1 can be dosed to animal by IP injection at 110 mg/Kg once per day to get antitumor responses.


  • 1. Jayathilaka K, et al. A chemical compound that stimulates the human homologous recombination protein RAD51. (2008) Proc Natl Acad Sci USA. 105(41):15848-53.
  • 2. Mason JM, et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. (2014) Cancer Res. 74(13):3546-55. 
  • 3. Song J, et al. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. (2016) Nat Commun. 7:10548. 

Products are for research use only. Not for human use. 

Payment & Security

American Express Apple Pay Diners Club Discover Elo Google Pay JCB Mastercard PayPal Shop Pay Venmo Visa

Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.

Estimate shipping

You may also like

Recently viewed