1M7

1M7 is a reagent for RNA SHAPE-MaP experiments that enables analysis of RNA secondary structures at single nucleotide resolution in <70 seconds.

Product information

CAS Number: 73043-80-8

Molecular Weight: 222.15

Formula: C9H6N2O5

Synonym:

1-methyl-7-nitroisatoic anhydride

1-methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione

1-methyl-7-nitro-1H-benzo[d][1,3]oxazine-2,4-dione

Chemical Name: 1-Methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione

Smiles: CN1C2=CC(=CC=C2C(=O)OC1=O)[N+]([O-])=O

InChiKey: MULNCJWAVSDEKJ-UHFFFAOYSA-N

InChi: InChI=1S/C9H6N2O5/c1-10-7-4-5(11(14)15)2-3-6(7)8(12)16-9(10)13/h2-4H,1H3

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in DMSO.

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

References:

1. Mortimer et al (2007) A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry. J.Am.Chem.Soc. 129 4144 PMID: 17367143.
2. Turner et al (2013) Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7). RNA 19 1857 PMID: 24141619.
3. Siegfried et al (2014) RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP). Nat.Methods 11 959 PMID: 25028896.
4. Smola et al (2015) Detection of RNA-Protein interactions in living cells with SHAPE. Biochemistry 54 6867 PMID: 26544910.

Products are for research use only. Not for human use.