Necrosulfonamide

Necrosulfonamide is a very specific and potent necrosis inhibitor and blocks mixed lineage kinase domain-like protein (MLKL).

Product information

CAS Number: 1360614-48-7

Molecular Weight: 461.47

Formula: C18H15N5O6S2

Synonym:

MLKL inhibitor

Necrosis Inhibitor III

Necrosulfonamide

Mixed Lineage Kinase Domain-Like Protein Inhibitor

Necrosome Inhibitor II

Chemical Name: N-(4-(N-(3-Methoxypyrazin-2-yl)sulfamoyl)phenyl)-3-(5-nitrothiophene-2-yl)acrylamide

Smiles: COC1=NC=CN=C1NS(=O)(=O)C1C=CC(=CC=1)NC(=O)C=CC1=CC=C(S1)[N+]([O-])=O

InChiKey: FNPPHVLYVGMZMZ-XBXARRHUSA-N

InChi: InChI=1S/C18H15N5O6S2/c1-29-18-17(19-10-11-20-18)22-31(27,28)14-6-2-12(3-7-14)21-15(24)8-4-13-5-9-16(30-13)23(25)26/h2-11H,1H3,(H,19,22)(H,21,24)/b8-4+

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in 1:50 DMF:PBS (pH 7.2); DMSO; DMF

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

Necrosulfonamide specifically blocks necrosis downstream of RIP3 activation. Necrosulfonamide inhibits MLKL-mediated necrosis by blocking Its N-terminal CC domain function.

References:

1. Chen S, Lv X, Hu B, Shao Z, Wang B, Ma K, Lin H, Cui M. RIPK1/RIPK3/MLKL-mediated necroptosis contributes to compression-induced rat nucleus pulposus cells death. Apoptosis. 2017 May;22(5):626-638. doi: 10.1007/s10495-017-1358-2. PubMed PMID: 28289909.
2. Zhao W, Feng H, Sun W, Liu K, Lu JJ, Chen X. Tert-butyl hydroperoxide (t-BHP) induced apoptosis and necroptosis in endothelial cells: Roles of NOX4 and mitochondrion. Redox Biol. 2017 Apr;11:524-534. doi: 10.1016/j.redox.2016.12.036. Epub 2017 Jan 5. PubMed PMID: 28088644; PubMed Central PMCID: PMC5237803.
3. Filipczak PT, Thomas C, Chen W, Salzman A, McDonald JD, Lin Y, Belinsky SA. TSC2 Deficiency Unmasks a Novel Necrosis Pathway That Is Suppressed by the RIP1/RIP3/MLKL Signaling Cascade. Cancer Res. 2016 Dec 15;76(24):7130-7139. Epub 2016 Oct 18. PubMed PMID: 27756752.

Products are for research use only. Not for human use.