Ionomycin


Catalog No. size PriceQuantity
M6780-2 2mg solid $722
M6780-10 10mg solid $2,876

Description

Cas:56092-81-0(free acid)

Product Information

Ionomycin Free Acid is a Ca 2+ ionophore. Its depletion of intracellular Ca 2+ stores may be linked to the induction of apoptosis.

 

Chemical Formula: C41H72O9

 

Exact Mass: 708.5176

 

Molecular Weight: 709.01

 

Elemental Analysis: C, 69.46; H, 10.24; O, 20.31

 

Related CAS #: 56092-81-0 (free acid)   56092-82-1 (calcium)    

 

Synonym:

 

Ionomycin

 

Chemical Name:

(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-trihydroxy-22-((2S,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-9-oxodocosa-10,16-dienoic acid

 

InChi Key:

PGHMRUGBZOYCAA-OJFQOPKISA-N

 

InChi Code:

InChI=1S/C41H72O9/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47)/b13-11+,34-24-/t25-,26-,27+,28-,29-,30+,31+,32-,33?,36+,37?,39-,40+,41+/m1/s1

 

Smiles Code:

C[C@@H](C[C@@H](C[C@@H](C(/C=C(O)/[C@@H](C[C@@H](C/C=C/[C@H]([C@@H](O)[C@H]([C@@H](O)CC1CC[C@@](C)(C2CC[C@@](C)([C@H](O)C)O2)O1)C)C)C)C)=O)C)C)CCC(O)=O

 

 

Technical Data:

 

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

 

In Vitro

Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus.

Addition of 2 μM Ionomycin to LCLC 103H cells causes an instantaneous increase in intracellular Ca2+ concentration from 50 to 180 nM. DNA and protein analysis in Ionomycin-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typical of caspase-mediated apoptosis. Necrosis could be detected in ~1-5% of the Ionomycin treated cells. Caspase activation in whole cells was followed by monitoring the increase in activity against Ac-DEVD-amc following Ionomycin treatment.

Ionomycin-mediated cleavage and exosome release. Following Ionomycin exposure, medium conditioned by SKOV3ip cells had increased amounts of exosomes containing the L1-32 cleavage fragment.

 

 

References

 

  1. Okudera M, Gojoubori T, Tsujino I, Asano M. Effect of ionomycin on interaction of calnexin with vesicular stomatitis virus glycoprotein is cell type-specific. J Oral Sci. 2015;57(4):305-12. doi: 10.2334/josnusd.57.305. PubMed PMID: 26666853.

 

  1. Nakamura S, Nakanishi A, Takazawa M, Okihiro S, Urano S, Fukui K. Ionomycin-induced calcium influx induces neurite degeneration in mouse neuroblastoma cells: analysis of a time-lapse live cell imaging system. Free Radic Res. 2016;50(11):1214-1225. Epub 2016 Sep 29. PubMed PMID: 27573976.

 

  1. Zhang Z, Zhang Y, Mou Z, Chu S, Chen X, He W, Guo X, Yuan Y, Takahashi M, Chen N. Tyrosine 402 phosphorylation of Pyk2 is involved in ionomycin-induced neurotransmitter release. PLoS One. 2014 Apr 9;9(4):e94574. doi: 10.1371/journal.pone.0094574. eCollection 2014. PubMed PMID: 24718602; PubMed Central PMCID: PMC3981813.

 

  1. Zheng W, Feng X, Qiu L, Pan Z, Wang R, Lin S, Hou D, Jin L, Li Y. Identification of the antibiotic ionomycin as an unexpected peroxisome proliferator-activated receptor γ (PPARγ) ligand with a unique binding mode and effective glucose-lowering activity in a mouse model of diabetes. Diabetologia. 2013 Feb;56(2):401-11. doi: 10.1007/s00125-012-2777-9. Epub 2012 Nov 23. PubMed PMID: 23178929.

 

  1. Nakazawa S, Shirae-Kurabayashi M, Otsuka K, Sawada H. Proteomics of ionomycin-induced ascidian sperm reaction: Released and exposed sperm proteins in the ascidian Ciona intestinalis. Proteomics. 2015 Dec;15(23-24):4064-79. doi: 10.1002/pmic.201500162. Epub 2015 Sep 25. PubMed PMID: 26223815.

 

Products are for research use only. Not for human use.

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