Ionomycin calcium


Catalog No. size PriceQuantity
M6798-2 2mg solid $137
M6798-10 10mg solid $577

Description

Cas:56092-82-1 (calcium)

Product Information

Ionomycin calcium is a polyether Ca 2+ ionophore. Its depletion of intracellular Ca 2+ stores may be linked to the induction of apoptosis.

 

Chemical Formula: C41H70CaO9

 

Exact Mass: 746.4646

 

Molecular Weight: 747.08

 

Elemental Analysis: C, 65.92; H, 9.44; Ca, 5.36; O, 19.27

 

Related CAS #: 56092-81-0 (free acid)   56092-82-1 (calcium)    

 

Synonym:

 

onomycin

calcium salt

 

Chemical Name:

Calcium(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-19,21-dihydroxy-22-((2S,2'R,5S,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate

 

InChi Key:

WKRWUYKLUMMAKG-WYGBAUISSA-L

 

InChi Code:

InChI=1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

 

Smiles Code:

C[C@@H](C[C@@H](C[C@@H](C(/C=C([O-])/[C@@H](C[C@@H](C/C=C/[C@H]([C@@H](O)[C@H]([C@@H](O)C[C@@H]1CC[C@@](C)([C@H]2CC[C@@](C)([C@H](O)C)O2)O1)C)C)C)C)=O)C)C)CCC([O-])=O.[Ca+2]

 

 

Technical Data:

 

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

 

In Vitro:

Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus.

Addition of 2 μM Ionomycin to LCLC 103H cells causes an instantaneous increase in intracellular Ca2+ concentration from 50 to 180 nM. DNA and protein analysis in Ionomycin-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typical of caspase-mediated apoptosis. Necrosis could be detected in ~1-5% of the Ionomycin treated cells. Caspase activation in whole cells was followed by monitoring the increase in activity against Ac-DEVD-amc following Ionomycin treatment.

Ionomycin-mediated cleavage and exosome release. Following Ionomycin exposure, medium conditioned by SKOV3ip cells had increased amounts of exosomes containing the L1-32 cleavage fragment.

 

 

References

 

1. Nakamura S, Nakanishi A, Takazawa M, Okihiro S, Urano S, Fukui K. Ionomycin-induced calcium influx induces neurite degeneration in mouse neuroblastoma cells: analysis of a time-lapse live cell imaging system. Free Radic Res. 2016;50(11):1214-1225. Epub 2016 Sep 29. PubMed PMID: 27573976.

 

2. Müller MS, Obel LF, Waagepetersen HS, Schousboe A, Bak LK. Complex actions of ionomycin in cultured cerebellar astrocytes affecting both calcium-induced calcium release and store-operated calcium entry. Neurochem Res. 2013 Jun;38(6):1260-5. doi: 10.1007/s11064-013-1021-4. Epub 2013 Mar 22. PubMed PMID: 23519933.

 

3. Lee WK, Dittmar T. Cytosolic calcium measurements in renal epithelial cells by flow cytometry. J Vis Exp. 2014 Oct 28;(92):e51857. doi: 10.3791/51857. PubMed PMID: 25407650; PubMed Central PMCID: PMC4353377.

 

4. Chatila T, Silverman L, Miller R, Geha R. Mechanisms of T cell activation by the calcium ionophore ionomycin. J Immunol. 1989 Aug 15;143(4):1283-9. PubMed PMID: 2545785.

 

5. Vasilev F, Chun JT, Gragnaniello G, Garante E, Santella L. Effects of ionomycin on egg activation and early development in starfish. PLoS One. 2012;7(6):e39231. doi: 10.1371/journal.pone.0039231. Epub 2012 Jun 18. PubMed PMID: 22723970; PubMed Central PMCID: PMC3377674.

 

6. Campbell LE, Nelson J, Gibbons E, Judd AM, Bell JD. Membrane properties involved in calcium-stimulated microparticle release from the plasma membranes of S49 lymphoma cells. ScientificWorldJournal. 2014 Jan 21;2014:537192. doi: 10.1155/2014/537192. eCollection 2014. PubMed PMID: 24578641; PubMed Central PMCID: PMC3918691.

 

Products are for research use only. Not for human use.

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