Description
Cas:1206711-16-1
Product Information:
SJ000291942 is a bone morphogenic protein (BMP) signaling activator.
Chemical Formula: C16H15FN2O4
Exact Mass: 318.1016
Molecular Weight: 318.3044
Elemental Analysis: C, 60.37; H, 4.75; F, 5.97; N, 8.80; O, 20.11
Chemical Name:
2-(4-ethylphenoxy)-N-(4-fluoro-3-nitrophenyl)-acetamide
InChi Key:
YSZJLXPXVFGTNK-UHFFFAOYSA-N
InChi Code: InChI=1S/C16H15FN2O4/c1-2-11-3-6-13(7-4-11)23-10-16(20)18-12-5-8-14(17)15(9-12)19(21)22/h3-9H,2,10H2,1H3,(H,18,20)
Smiles Code:
O=C(NC1=CC=C(F)C([N+]([O-])=O)=C1)COC2=CC=C(CC)C=C2
Products are for research use only. Not for human use.
Technical Data:
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
In Vitro:
Embryos treated with SJ000291942 display the most severe ventralization and SJ000291942 is also the most potent. SJ000291942 also causes more mortality, and at lower doses than controls and the other two compounds. This demonstrates our compounds cause ventralization of embryos consistent with increased BMP signaling activity. SJ000291942 causes an increase in bmp2b and szl expression. Zebrafish assays suggest that SJ000291942 activates the canonical BMP signaling pathway. To extend these observations, immunoblotting of protein lysates from C33A-2D2 cells stimulated with SJ000291942 at different times is performed. SJ000291942 activates phosphorylation of SMAD1/5/8 in serum-free medium. Like in zebrafish embryos, SJ000291942 is most active. SJ000291942 induces p-SMAD1/5/8 maximally at 1hr of treatment. Immunoblotting analysis of lysates from C33A-2D2 treated with SJ000291942 reveals clear induction of the phosphorylated Extracellular Signal-regulated protein Kinase, ERK1/2 (P-ERK1/2) by SJ000291942. The highest dose (100 and 300ng) BMP4 treatments generate a gene expression signature most similar to osteoblast expression. Low dose (10ng) BMP4 treatment aligns closely with 25μM compound 3 treatment and with 25μM SJ000291942.
References:
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Genthe, J.R., Min, J., Farmer, D.M., et al. Ventromorphins: A new class of small molecule activators of the canonical BMP signaling pathway. ACS Chem. Biol. 12(9), 2436-2447 (2017).
Products are for research use only. Not for human use.
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