RAMB4

Cas：145888-79-5

Product Information：

RAMB4, also known as PTP1B-IN-9, is a ubiquitin-proteasome system (UPS)-stressor. RAMB4 selectively reduces the viability of cervical cancer cells independently of HPV genotype via blockade of proteasomal degradation. RAMB4 treatment triggers a Ubiquitin-Proteasome-System (UPS)-stress response without affecting 20S proteasome catalytic activities.

Chemical Formula: C19H13Cl4NO

Exact Mass: 410.9751

Molecular Weight: 413.119

Elemental Analysis: C, 55.24; H, 3.17; Cl, 34.32; N, 3.39; O, 3.87

Synonym:

PTP1B-IN-9

PTP1B-IN 9

PTP1B-IN9

RAMB4

RAMB-4

RAMB 4

Chemical Name: 

(3E,5E)-3,5-Bis(3,4-dichlorobenzylidene)piperidin-4-one

InChi Key:

GJPXGFGIFQWUOC-ACFHMISVSA-N

InChi Code: InChI=1S/C19H13Cl4NO/c20-15-3-1-11(7-17(15)22)5-13-9-24-10-14(19(13)25)6-12-2-4-16(21)18(23)8-12/h1-8,24H,9-10H2/b13-5+,14-6+

Smiles Code:

O=C1/C(CNC/C1=C\C2=CC=C(Cl)C(Cl)=C2)=C/C3=CC=C(Cl)C(Cl)=C3

Technical Data：

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

In Vitro:

PTP1B-IN-9 (0-30 µM; 48 hours) treatment produces a dose dependent reduction in the viability of HPV16-positive SiHa and Caski cells and HPV-39-positive ME180 cervical cancer cell lines respectively.

PTP1B-IN-9 reduces the cell viability of exponentially growing HeLa cervical cancer cells in a dose-dependent fashion with an IC50 value of 2 µM.

References：

1. Anchoori RK, et al. Stressing the ubiquitin-proteasome system without 20S proteolytic inhibition selectively kills cervical cancer cells.PLoS One. 2011;6(8):e23888.

Products are for research use only. Not for human use.