Sulfo-NHS-LC-Biotin

Sulfo-NHS-LC-Biotin is a water-soluble amine reactive biotinylation reagent used to attach biotin to primary amines. It is commonly used to label proteins and antibodies and does not penetrate membrane. It is reactive in pH 7.0-9.0 and has a solubility of 10 mg/mL in water and DMF.

Product information

CAS Number: 191671-46-2

Molecular Weight: 556.59

Formula: C20H29N4NaO9S2

Synonym:

Sulfo NHS LC Biotin

Sulfo-NHS-LC-Biotin

Chemical Name: sodium2,5-dioxo-1-((6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoyl)oxy)pyrrolidine-3-sulfonate

Smiles: [Na+].[O-]S(=O)(=O)C1CC(=O)N(OC(=O)CCCCCNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23)C1=O

InChiKey: JJGWLCLUQNFDIS-GTSONSFRSA-M

InChi: InChI=1S/C20H30N4O9S2.Na/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32;/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32);/q;+1/p-1/t12-,13-,14?,18-;/m0./s1

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in DMSO

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

References:

1. Huang L, Bao H, Li S, Zhang J, Li L, Zhang B, Yu Y, Liu Y, Li H. Improvement of the sensitivity of the detection of Gal d 6-specific IgE via biotinylation in vivo. Allergol Immunopathol (Madr). 2020 Jul-Aug;48(4):348-354. doi: 10.1016/j.aller.2020.02.002. Epub 2020 Jun 10. PMID: 32532469.
2. Wu HN, Miyata M. Whole surface image of Mycoplasma mobile, suggested by protein identification and immunofluorescence microscopy. J Bacteriol. 2012 Nov;194(21):5848-55. doi: 10.1128/JB.00976-12. Epub 2012 Aug 24. PMID: 22923591; PMCID: PMC3486091.

Products are for research use only. Not for human use.