DOPE

Liposome/lipoplex transfection reagent. Enhances liposome and lipoplex transfection efficiency in combination with DA, DOTAP or DC-cholesterol (Cat. No. 7176). Please note: exact ratio for use and transfection efficiency is dependent on cell line used.

Product information

CAS Number: 4004-05-1

Molecular Weight: 744.03

Formula: C41H78NO8P

Synonym:

1, 2-DOPE

1, 2-Dioleoyl-sn-glycero-3-Phosphoethanolamine

Chemical Name: 2-Aminoethyl (R)-2,3-Bis(oleoyloxy)propyl Hydrogen Phosphate

Smiles: CCCCCCCC/C=C\CCCCCCCC(=O)O[C@@H](COP(O)(=O)OCCN)COC(=O)CCCCCCC/C=C\CCCCCCCC

InChiKey: MWRBNPKJOOWZPW-NYVOMTAGSA-N

InChi: InChI=1S/C41H78NO8P/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-31-33-40(43)47-37-39(38-49-51(45,46)48-36-35-42)50-41(44)34-32-30-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h17-20,39H,3-16,21-38,42H2,1-2H3,(H,45,46)/b19-17-,20-18-/t39-/m1/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 31.25 mg/mL (42.00 mM; Need ultrasonic) Ethanol : ≥ 25 mg/mL (33.60 mM)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

In this study, each siRNA is encapsulated in LNP (cationic lipid-A: PEG2000-DMPE: DOPE = 47.8: 5.18: 47.1 molar percent). With the neutral lipid for the LNP is DOPE, LNP exerts knock-down (KD) effecton macrophages and DCs and Liposome-containing DOPE shows fusogenicity. LNP/siRNA significantly suppresses CD45 protein expression at an inhibition rate of 30% on macrophages and DCs but not on monocytes, neutrophils, plasmacytoid DCs, or B cells. LNP has the potential to be an efficient siRNA delivery system not only for murine but also for human macrophages and DCs.

References:

1. Uemura Y, et al. The efficiency of lipid nanoparticles with an original cationic lipid as a siRNA delivery system for macrophages and dendritic cells.Pharm Dev Technol. 2019 Mar;24(3):263-268.

Products are for research use only. Not for human use.