HM Janelia Fluor® 526, SE is a spontaneously blinking green-emitting dye and a hydroxymethyl derivative of JF526. It is suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. It is also suitable for conventional confocal microscopy and live cell imaging. Fluorogenic spontaneously blinking green-emitting dye; hydroxymethyl derivative of JF526. Suitable for super-resolution imaging including dSTORM, STED and single-molecule localization spectroscopy (SMLSM). The spontaneous blinking properties of HM Janelia Fluor® 526, avoids the requirement for strongly reducing dSTORM buffers. Also suitable for conventional confocal microscopy and live cell imaging. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. To measure the absorbance spectrum of this dye we recommend the following solvent: TFE plus 0.1% TFA.
CAS Number: 2376841-30-2
Molecular Weight: 645.51
HM JF526 NHS
Chemical Name: 2,5-dioxopyrrolidin-1-yl 3',6'-bis(3,3-difluoroazetidin-1-yl)-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxylate
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: Soluble in DMSO
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
Products are for research use only. Not for human use.
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