Indirubin

Indirubin is a potent and selective CDK inhibitor. Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin has been used to treat the symptoms of leukemia. Further biological explorations revealed the ability of indirubin to bind cyclin-dependent kinases and 6-bromoindirubin, extracted from mollusks, to bind glycogen synthase kinase-3. Studies have shown that Indirubin can help keep cancer cells from reproducing in rats. Indirubin also appears to reduce inflammation by inhibiting part of the immune response.

Product information

CAS Number: 479-41-4

Molecular Weight: 262.26

Formula: C16H10N2O2

Synonym:

C.I. 73200

Couroupitine B

Indigo red

Indigopurpurin

NSC 105327

NSC-105327

NSC105327

Chemical Name: 3-(1, 3-Dihydro-3-oxo-2H-indol-2-ylidene)-1, 3-dihydro-2H-indol-2-one

Smiles: O=C1NC2=CC=CC=C2/C/1=C1/NC2=CC=CC=C2C/1=O

InChiKey: CRDNMYFJWFXOCH-YPKPFQOOSA-N

InChi: InChI=1S/C16H10N2O2/c19-15-10-6-2-4-8-12(10)17-14(15)13-9-5-1-3-7-11(9)18-16(13)20/h1-8,17H,(H,18,20)/b14-13-

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO: 53 mg/mL(202.08 mM). Water: Insoluble.

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin inhibits CDKs activity with IC50 of 2.2 - 10 μM resulting in cell cycle arrest in the G2/M phase. Indirubin also inhibits GSK-3β with an IC50 of 0.6 μM, attenuating CDK5- and GSK-3β-mediated tau phosphorylation, a process over-active in Alzheimer disease states. It also suppresses tumor necrosis factor (TNF)-induced NF-κB activation in a dose- and time-dependent manner. Indirubin also suppresses the NF-κB activation induced by various inflammatory agents and carcinogens. Indirubin blocks the phosphorylation and degradation of IκBα through the inhibition of activation of IκBα kinase and phosphorylation and nuclear translocation of p65.

References:

1. Sethi G, et al. J Biol Chem, 2006, 281(33), 23425-2335.
2. Leclerc S, et al. J Biol Chem, 2001, 276(1), 251-260.
3. Hoessel R, et al. Nat Cell Biol, 1999, 1(1), 60-67.

Products are for research use only. Not for human use.