Doxycycline Hyclate is the hyclate salt form of a synthetic, broad-spectrum tetracycline antibiotic exhibiting antimicrobial activity. It is an antimicrobial tetracycline that acts as an inhibitor of a wide range of matrix metalloproteinases (MMPs) of MMP-1 and MMP-8. Doxycycline hyclate binds reversibly to the 30S ribosomal subunit, possibly to the 50S ribosomal subunit as well, thereby blocking the binding of aminoacyl-tRNA to the mRNA-ribosome complex. This leads to an inhibition of protein synthesis.
CAS Number: 24390-14-5
Molecular Weight: 512.94
Doxycycline hydrochloride hemiethanolate hemihydrate
Chemical Name: (4S,4aR,5S,5aR,6R,12aS)-4-(dimethylamino)-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-1,4,4a,5,5a,6,11,12a-octahydrotetracene-2-carboxamide ethanol hydrate hydrochloride
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 240 mg/mL (467.89 mM; Need ultrasonic) H2O : 13.89 mg/mL (27.08 mM; Need ultrasonic)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
Doxycycline (hyclate) (Doxycycline hydrochloride hemiethanolate hemihydrate) affects growth of glioma cells only under high concentrations. Doxycycline (hyclate) decreases MT-CO1 protein content with concentrations of 1 µg/mL and higher in SVG cells. Doxycycline (1 µg/mL) induce short-hairpin RNA (shRNA) expression in H23 cells.
Doxycycline (hyclate) (Doxycycline hydrochloride hemiethanolate hemihydrate) (oral; 200 or 800 mg/kg/day; for 3 months) reduces active MMP-9 in untreated HT mice in a dose-dependent manner.
- Wilfried Briest, et al. Doxycycline ameliorates the susceptibility to aortic lesions in a mouse model for the vascular type of Ehlers-Danlos syndrome. J Pharmacol Exp Ther. 2011 Jun;337(3):621-7.
- Luger AL, et al. Doxycycline Impairs Mitochondrial Function and Protects Human Glioma Cells from Hypoxia-Induced Cell Death: Implications of Using Tet-Inducible Systems. Int J Mol Sci. 2018 May 17;19(5).
- Eusebio Manchado, et al. A combinatorial strategy for treating KRAS-mutant lung cancer. Nature. 2016 Jun 30;534(7609):647-51.
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.