Catalog No. size PriceQuantity
S10003 1000X; 0.5 ml $79


Product Information
Purity: ≥98% for each compound
Storage: 4oC for 1 month.
-20oC for 3 month.
Cocktail Formulation: 3 mM CHIR99021 and 2 mM SB431542 in DMSO solution Effective Concentration in Cell Culture: 3 µM CHIR99021 and 2 µM SB431542 in cell culture medium


Biological Activity:

 A major challenge of the current hESC/iPSC differentiation paradigm is the inability to effectively capture and long-term, stably expand lineage-specific stem cells that retain broad differentiation potential and, more importantly, developmental stage-specific differentiation propensity. To overcome this challenge, the combination of a GSK3 inhibitor (CHIR99021, 3 µM) and a TGFβ receptor inhibitor (SB431542, 2 µM), pNSC-CSB, was developed to be used alone or further combined with a Notch pathway inhibitor to rapidly and efficiently convert hESCs/iPSCs into a homogenous population of primitive neural stem cells  (pNSCs) within 7 days under chemically defined conditions. Most importantly, these pNSCs were shown to stably self-renew in the presence of leukemia inhibitory factor/LIF and the small molecule combination of CHIR99021 and SB431542 (pNSC-CSB). Under this condition, long-term expanded pNSCs retain high neurogenic potential and responsiveness to instructive patterning cues toward midbrain and hindbrain neuronal subtypes, and exhibit in vivo integration.

How to Use:


  • In vitro: Dilute received Ready-to-use kit pNSC-CSB at 1:1000 into your hESC/iPSC neural differentiation basal media to make the pNSC-CSB media. The reconstituted pNSC-CSB media can be used alone to initiate primitive neuroepithelium induction for 7-10 days, and the generated pNSCs can then be expanded and passaged in the pNSC-CSB media further supplemented with LIF. 



  1. Li W, et al. Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors. (2011) PNAS. 108(20):8299-304.

Products are for research use only. Not for human use.

Payment & Security

PayPal Venmo

Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.

Estimate shipping

You may also like

Recently viewed