3-Chlorodiphenylamine

3-Chlorodiphenylamine is a high affinity Ca2+ sensitizer of cardiac muscle. 3-Chlorodiphenylamine is based on diphenylamine and binds to the isolated N-domain of cardiac troponin C (cTnC) (Kd=6 µM). 3-Chlorodiphenylamine is an excellent starting scaffold for the development of more potent Ca2+-sensitizing compounds due to its small size, and can be used for systolic heart failure research.

Product information

CAS Number: 101-17-7

Molecular Weight: 203.67

Formula: C12H10ClN

Chemical Name: 3-chloro-N-phenylaniline

Smiles: ClC1=CC(=CC=C1)NC1C=CC=CC=1

InChiKey: OHHIBZKYXJDQEU-UHFFFAOYSA-N

InChi: InChI=1S/C12H10ClN/c13-10-5-4-8-12(9-10)14-11-6-2-1-3-7-11/h1-9,14H

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

3-Chlorodiphenylamine is able to bind highly (Kd=10 µM) to a chimeric protein consisting of the regulatory N-domain of cTnC (cNTnC) and the switch region of cTnI (cNTnC–cSp chimera). 3-Chlorodiphenylamine (100 µM) results in a 1.5-fold increase in the Ca2+ sensitivity of force development without altering the maximal or resting forces in skinned ventricular trabeculae. 3-Chlorodiphenylamine (25-100 µM) increases Ca2+ sensitivity of the N-domain of intact cTnC after reconstitution into the cTn complex (cTnC complexed with cTnI and cTnT) in a concentration-dependent manner. It exhibits pCa50s with 6.39±0.01, 6.65±0.01, and 6.73±0.02 in the presence of 25, 50, and 100 µM 3-Chlorodiphenylamine, respectively.

Products are for research use only. Not for human use.