Ionomycin calcium

Ionomycin calcium is a polyether Ca 2+ ionophore. Its depletion of intracellular Ca 2+ stores may be linked to the induction of apoptosis.

Product information

CAS Number: 56092-82-1

Molecular Weight: 747.07

Formula: C41H70CaO9

Synonym:

Ionomycin calcium salt

onomycin

calcium salt

Chemical Name: Calcium(4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-19,21-dihydroxy-22-((2S,2'R,5S,5'S)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-11-oxido-9-oxodocosa-10,16-dienoate

Smiles: [Ca+2].C[C@@H](O)[C@]1(C)CC[C@@H](O1)[C@]1(C)CC[C@@H](C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C/C[C@@H](C)C[C@@H](C)/C(/[O-])=C/C(=O)[C@@H](C)C[C@@H](C)C[C@H](C)CCC([O-])=O)O1

InChiKey: WKRWUYKLUMMAKG-WYGBAUISSA-L

InChi: InChI=1S/C41H72O9.Ca/c1-25(21-29(5)34(43)24-35(44)30(6)22-27(3)20-26(2)14-15-38(46)47)12-11-13-28(4)39(48)31(7)36(45)23-33-16-18-41(10,49-33)37-17-19-40(9,50-37)32(8)42;/h11,13,24-33,36-37,39,42-43,45,48H,12,14-23H2,1-10H3,(H,46,47);/q;+2/p-2/b13-11+,34-24-;/t25-,26-,27+,28-,29-,30+,31+,32-,33+,36+,37-,39-,40+,41+;/m1./s1

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in DMSO

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

Ionomycin is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus. Addition of 2 μM Ionomycin to LCLC 103H cells causes an instantaneous increase in intracellular Ca2+ concentration from 50 to 180 nM. DNA and protein analysis in Ionomycin-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typical of caspase-mediated apoptosis. Necrosis could be detected in ~1-5% of the Ionomycin treated cells. Caspase activation in whole cells was followed by monitoring the increase in activity against Ac-DEVD-amc following Ionomycin treatment. Ionomycin-mediated cleavage and exosome release. Following Ionomycin exposure, medium conditioned by SKOV3ip cells had increased amounts of exosomes containing the L1-32 cleavage fragment.

References:

1. Nakamura S, Nakanishi A, Takazawa M, Okihiro S, Urano S, Fukui K. Ionomycin-induced calcium influx induces neurite degeneration in mouse neuroblastoma cells: analysis of a time-lapse live cell imaging system. Free Radic Res. 2016;50(11):1214-1225. Epub 2016 Sep 29. PubMed PMID: 27573976.
2. Müller MS, Obel LF, Waagepetersen HS, Schousboe A, Bak LK. Complex actions of ionomycin in cultured cerebellar astrocytes affecting both calcium-induced calcium release and store-operated calcium entry. Neurochem Res. 2013 Jun;38(6):1260-5. doi: 10.1007/s11064-013-1021-4. Epub 2013 Mar 22. PubMed PMID: 23519933.
3. Lee WK, Dittmar T. Cytosolic calcium measurements in renal epithelial cells by flow cytometry. J Vis Exp. 2014 Oct 28;(92):e51857. doi: 10.3791/51857. PubMed PMID: 25407650; PubMed Central PMCID: PMC4353377.
4. Chatila T, Silverman L, Miller R, Geha R. Mechanisms of T cell activation by the calcium ionophore ionomycin. J Immunol. 1989 Aug 15;143(4):1283-9. PubMed PMID: 2545785.

Products are for research use only. Not for human use.