β-amyloid (12-28) (TFA)

β-amyloid (12-28) TFA is a peptide fragment of β-amyloid protein (β1-42). β1–42 a 42 amino acid protein , is the major component of senile plaque cores. β-amyloid (12-28) shows aggregation properties. β-amyloid (12-28) has the potential for Alzheimer’s disease research.

Product information

Molecular Weight: 2069.20

Formula: C91H136F3N25O27

Chemical Name: (2S, 5S, 8S, 14S, 17S, 20S)-5-(2-amino-2-oxoethyl)-2-(4-aminobutyl)-20-((5S, 8S, 11S, 14S, 17S, 20S, 23S, 26S, 29S)-23, 26-bis((1H-imidazol-4-yl)methyl)-29-amino-20-(3-amino-3-oxopropyl)-17-(4-aminobutyl)-5, 8-dibenzyl-14-isobutyl-11-isopropyl-2, 30-dimethyl-4, 7, 10, 13, 16, 19, 22, 25, 28-nonaoxo-3, 6, 9, 12, 15, 18, 21, 24, 27-nonaazahentriacontanamido)-17-(carboxymethyl)-8-(hydroxymethyl)-14-isopropyl-4, 7, 10, 13, 16, 19-hexaoxo-3, 6, 9, 12, 15, 18-hexaazatricosane-1, 23-dioic acid compound with 2, 2, 2-trifluoroacetic acid (1:1)

Smiles: CC(NC(=O)[C@H](CC1C=CC=CC=1)NC(=O)[C@H](CC1C=CC=CC=1)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC1=CNC=N1)NC(=O)[C@H](CC1=CNC=N1)NC(=O)[C@@H](N)C(C)C)C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O.OC(=O)C(F)(F)F

InChiKey: LGFUGDGSFHJOQB-HJXDCQBZSA-N

InChi: InChI=1S/C89H135N25O25.C2HF3O2/c1-45(2)32-58(106-75(124)54(24-16-18-30-90)103-76(125)55(26-28-66(92)116)104-80(129)61(35-52-39-95-43-98-52)108-81(130)62(36-53-40-96-44-99-53)111-86(135)71(94)46(3)4)83(132)114-73(48(7)8)88(137)112-60(34-51-22-14-11-15-23-51)79(128)107-59(33-50-20-12-10-13-21-50)78(127)100-49(9)74(123)102-56(27-29-69(119)120)77(126)110-64(38-70(121)122)84(133)113-72(47(5)6)87(136)97-41-68(118)101-65(42-115)85(134)109-63(37-67(93)117)82(131)105-57(89(138)139)25-17-19-31-91;3-2(4,5)1(6)7/h10-15,20-23,39-40,43-49,54-65,71-73,115H,16-19,24-38,41-42,90-91,94H2,1-9H3,(H2,92,116)(H2,93,117)(H,95,98)(H,96,99)(H,97,136)(H,100,127)(H,101,118)(H,102,123)(H,103,125)(H,104,129)(H,105,131)(H,106,124)(H,107,128)(H,108,130)(H,109,134)(H,110,126)(H,111,135)(H,112,137)(H,113,133)(H,114,132)(H,119,120)(H,121,122)(H,138,139);(H,6,7)/t49?,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-,65-,71-,72-,73-;/m0./s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 120 mg/mL (57.99 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

β-amyloid (12-28) may exert dysregulation cognitive effects by means of defective coordination of potassium channel function in nerve, glia and endothelial cells. β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure. 2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃. 3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing. 4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments. Methods vary depends on the downstream applications.

References:

1. Deiber JA, Peirotti MB, Piaggio MV. Global properties and propensity to dimerization of the amyloid-beta (12-28) peptide fragment through the modeling of its monomer and dimer diffusion coefficients and electrophoretic mobilities. Electrophoresis. 2015 Mar;36(5):805-12.

Products are for research use only. Not for human use.