8-Br-GTP

8-Br-GTP, a GTP analog, is a competitive FtsZ polymerization and GTPase activity (Ki of 31.8 μM) inhibitor. 8-Br-GTP can be used for nucleic acid modification.

Product information

CAS Number: 23197-98-0

Molecular Weight: 602.08

Formula: C10H15BrN5O14P3

Chemical Name: ({[({[(2R,3S,4R,5R)-5-(2-amino-8-bromo-6-oxo-6,9-dihydro-3H-purin-9-yl)-5-hydrogenio-3,4-dihydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl)oxy](hydroxy)phosphoryl}oxy)phosphonic acid

Smiles: NC1NC2=C(N=C(Br)N2[C@@H]2O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]2O)C(=O)N=1

InChiKey: CNYUHYBBHQMZQJ-UMMCILCDSA-N

InChi: InChI=1S/C10H15BrN5O14P3/c11-9-13-3-6(14-10(12)15-7(3)19)16(9)8-5(18)4(17)2(28-8)1-27-32(23,24)30-33(25,26)29-31(20,21)22/h2,4-5,8,17-18H,1H2,(H,23,24)(H,25,26)(H2,20,21,22)(H3,12,14,15,19)/t2-,4-,5-,8-/m1/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in DMSO

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

8-Br-GTP inhibits FtsZ polymerization but does not affect tubulin polymerization.8-Br-GTP has two stable orientations of the base toward the sugar: anti and syn. 8-Br-GTP shows preference for the syn conformation (population ~90%).

References:

1. Tilman Läppchen, et al. GTP analogue inhibits polymerization and GTPase activity of the bacterial protein FtsZ without affecting its eukaryotic homologue tubulin. Biochemistry. 2005 May 31;44(21):7879-84.
2. Jozef Hritz, et al. Optimization of replica exchange molecular dynamics by fast mimicking. J Chem Phys. 2007 Nov 28;127(20):204104.

Products are for research use only. Not for human use.