Rhosin hydrochloride

Rhosin (G04) hydrochloride (HCl) is a potent, specific inhibitor of RhoA subfamily Rho GTPases with Kd of ~ 0.4 uM. Rhosin hydrochloride induces cell apoptosis.

Product information

CAS Number: 1281870-42-5

Molecular Weight: 394.86

Formula: C20H19ClN6O

Chemical Name: D-Tryptophan (2E)-2-(6-quinoxalinylmethylene)hydrazide hydrochloride

Smiles: Cl.N[C@H](CC1=CNC2=CC=CC=C21)C(=O)N/N=C/C1=CC2=NC=CN=C2C=C1

InChiKey: SFRGBDFQSLZYLF-GRYLRVQNSA-N

InChi: InChI=1S/C20H18N6O.ClH/c21-16(10-14-12-24-17-4-2-1-3-15(14)17)20(27)26-25-11-13-5-6-18-19(9-13)23-8-7-22-18;/h1-9,11-12,16,24H,10,21H2,(H,26,27);1H/b25-11+;/t16-;/m1./s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO: 93 mg/mL(235.52 mM). Water: 93 mg/mL(235.52 mM).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

In cells Rhosin specifically inhibits RhoA activity and RhoA-mediated cellular function without affecting Cdc42 or Rac1 signaling activities. By suppressing RhoA or RhoC activity Rhosin can inhibit mammary sphere formation by breast cancer cells, suppress invasion of mammary epithelial cells, and induce neurite outgrowth of PC12 cells in synergy with NGF.

In Vivo:

Treatment with rhosin abrogates the lung metastasis of B16BL6 and 4T1 cells in vivo.

References:

1. Masanobu Tsubaki, et al. Biomedicines. 2021 Jan 4;9(1):35.
2. Xun Shang, et al. Chem Biol. 2012 Jun 22;19(6):699-710.

Products are for research use only. Not for human use.