Protein kinase affinity probe 1

Protein kinase affinity probe 1 is a novel protein kinase affinity probe for the functional identification of protein kinases (PKs). Protein kinase affinity probe 1 is a modified Purvalanol B (HY-18299) probe with 50% beads loading (Compound S3).

Product information

CAS Number: 2098621-90-8

Molecular Weight: 531.09

Formula: C26H39ClN8O2

Chemical Name: N-(6-aminohexyl)-2-chloro-4-[(2-{[(2R)-1-hydroxy-3-methylbutan-2-yl]amino}-9-(propan-2-yl)-9H-purin-6-yl)amino]benzamide

Smiles: CC(C)N1C=NC2C(NC3C=C(Cl)C(=CC=3)C(=O)NCCCCCCN)=NC(N[C@@H](CO)C(C)C)=NC1=2

InChiKey: DXPRFXZSNLVXII-NRFANRHFSA-N

InChi: InChI=1S/C26H39ClN8O2/c1-16(2)21(14-36)32-26-33-23(22-24(34-26)35(15-30-22)17(3)4)31-18-9-10-19(20(27)13-18)25(37)29-12-8-6-5-7-11-28/h9-10,13,15-17,21,36H,5-8,11-12,14,28H2,1-4H3,(H,29,37)(H2,31,32,33,34)/t21-/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

“S” indicates that the PKI (Purvalanol B) is sepharose-bound. In a series of pull-down experiments from the lysates of HeLa cells, the effects of 0.1, 0.5, 1, 2, 5, 10, 25, and 50% loadings of PK inhibitors is determined. Examine higher bead loadings (75 and 100%) are prevented due to lack of compound solubility and poor reproducibility of the synthesis outcomes. 50% loading is the highest feasible in this study.

References:

1. Deane FM, et al. FD5180, a Novel Protein Kinase Affinity Probe, and the Effect of Bead Loading on Protein Kinase Identification. ACS Omega. 2017 Jul 31;2(7):3828-3838.

Products are for research use only. Not for human use.