TAME is an inhibitor of anaphase-promoting complex/cyclosome (APC/C or APC), which binds to APC/C and prevents its activation by Cdc20 and Cdh1, produces mitotic arrest.
CAS Number: 901-47-3
Molecular Weight: 342.41
Tosylarginine methyl ester
N-4-Tosyl-L-arginine methyl ester
Related CAS Number:
1784-03-8 (TAME HCl)
Chemical Name: methyl (2S)-5-carbamimidamido-2-(4-methylbenzenesulfonamido)pentanoate
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO: 64 mg/mL (186.9 mM)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
TAME inhibits cyclin proteolysis in mitotic Xenopus egg extract with IC50 of 12 µM. TAME at concentration of 1-200 μM arrests interphase extract treated with recombinant cyclin B1/Cdc2 complex in mitosis, with stable cyclin B1 and phosphorylated Cdc27. TAME at concentration of 200 μM dramaticly inhibits the ubiquitin ligase activity of the Anaphase-Promoting Complex (APC), accompanied by reduced binding of Cdh1 to APC. TAME addition to interphase extract reduces Cdc20 association with the APC in a dose-dependent manner partly by binding directly to APC, and the contribution motif is the C-terminal isoleucine-arginine (IR) tail on APC.  TAME is hydrolysed by trypsin with Km of 0.328 mM.  TAME accelerates the ATP hydrolysis process about 12-fold.  TAME interacts with β and γ phosphate and the adenine ring of ATP by the guanidinium group and the aromatic ring.  TAME at concentration of 50 mM inhibits nutrient-induced germination and pressure-induced germination at 600 MPa in Bacillus subtilis.  TAME induces a concentration dependent contractile response on ileal strips with EC50 of 4.3 x 103 M.
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